KMID : 1025520150570010028
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Journal of Animal Science and Technology 2015 Volume.57 No. 1 p.28 ~ p.28
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Expression patterns of TR¥á and CRABPII genes in Chinese cashmere goat skin during prenatal development
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Zhong Tao
Zhao Wei Zhou Zhongqiang Li Li Wang Linjie Li Hua Zhang Hongping
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Abstract
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Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of TR¥á and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR).
Results: RT-qPCR showed that TR¥á was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of TR¥á mRNA was revealed at E130 (P?>?0.05). The expression pattern of CRABPII mRNA showed an ¡®up-down-up¡¯ trend, which revealed a significantly highest expression at E75 (P?0.05) and was down-regulated during E80 to E120 (P?0.05) and mildly increased at E130, subsequently.
Conclusion: This study demonstrated that TR¥á and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of TR¥á and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.
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KEYWORD
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Cashmere goat, TR¥á, CRABPII, Skin, Expression
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